Characterization of the transient species generated by the photoionization of Berberine: a laser flash photolysis study

Spectrochim Acta A Mol Biomol Spectrosc. 2009 Sep 1;73(5):955-9. doi: 10.1016/j.saa.2009.05.001. Epub 2009 May 9.

Abstract

Using 266 nm laser flash photolysis it has been demonstrated that Berberine (BBR) in aqueous solution is ionized via a mono-photonic process giving a hydrated electron, anion radical that formed by hydrated electron react with steady state of BBR, and neutral radical that formed from rapid deprotonation of the radical cation of BBR. The quantum yield of photoionization is determined to be 0.03 at room temperature with KI solution used as a reference. Furthermore utilizing pH changing method and the SO(4.)(-) radical oxidation method, the assignment of radical cation of BBR was further confirmed, the pK(a) value of it was calculated, and the related set up rate constant was also determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Berberine / chemistry*
  • Electrons
  • Free Radicals
  • Ions / chemistry
  • Lasers
  • Molecular Structure
  • Oxidation-Reduction
  • Photolysis*
  • Spectrophotometry, Ultraviolet
  • Sulfates / chemistry
  • Water / chemistry

Substances

  • Free Radicals
  • Ions
  • Sulfates
  • sulfate radical
  • Water
  • Berberine