Abstract
Every little drop: The K(D) values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein-protein interactions.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antibodies / immunology
-
Cells, Cultured
-
Fluorescence Resonance Energy Transfer / methods*
-
Fluorescent Dyes / chemistry
-
Humans
-
Kinetics
-
Microfluidics / instrumentation
-
Microfluidics / methods*
-
Protein Interaction Mapping / methods*
-
Ribonuclease, Pancreatic / analysis*
-
Ribonuclease, Pancreatic / immunology
-
Ribonuclease, Pancreatic / metabolism
-
Spectrometry, Fluorescence
-
Thermodynamics
Substances
-
Antibodies
-
Fluorescent Dyes
-
angiogenin
-
Ribonuclease, Pancreatic