Two new spirobiflavonoids from Abies chensiensis with moderate NO production inhibitory activity

Planta Med. 2009 Nov;75(14):1534-7. doi: 10.1055/s-0029-1185746. Epub 2009 Jun 4.

Abstract

Phytochemical investigation of the aerial parts of Abies chensiensis afforded two new (compounds 2 and 3) and 27 known compounds, including the related compound larixinol ( 1). The structures of spirobiflavonoids 1- 3 were established using 1D and 2D NMR spectroscopic techniques. In addition, the structure of larixinol ( 1) was confirmed by X-ray crystallographic analysis. Compounds 1- 3 were evaluated for inhibitory activities against LPS-induced NO production in macrophages. Larixinol ( 1) showed moderate effects, with an IC(50) value of 60.0 microg/mL. In addition, it did not show any cytotoxicity on RAW 264.7 macrophages at 100 microg/mL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abies / chemistry*
  • Animals
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line
  • Crystallography, X-Ray
  • Flavonoids / isolation & purification
  • Flavonoids / pharmacology*
  • Inhibitory Concentration 50
  • Lipopolysaccharides
  • Macrophages / drug effects*
  • Magnetic Resonance Spectroscopy
  • Mice
  • Nitric Oxide / biosynthesis*
  • Plant Components, Aerial
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Spiro Compounds / isolation & purification
  • Spiro Compounds / pharmacology*

Substances

  • Anti-Inflammatory Agents
  • Flavonoids
  • Lipopolysaccharides
  • Plant Extracts
  • Spiro Compounds
  • larixinol
  • Nitric Oxide