Abstract
Phytochemical investigation of the aerial parts of Abies chensiensis afforded two new (compounds 2 and 3) and 27 known compounds, including the related compound larixinol ( 1). The structures of spirobiflavonoids 1- 3 were established using 1D and 2D NMR spectroscopic techniques. In addition, the structure of larixinol ( 1) was confirmed by X-ray crystallographic analysis. Compounds 1- 3 were evaluated for inhibitory activities against LPS-induced NO production in macrophages. Larixinol ( 1) showed moderate effects, with an IC(50) value of 60.0 microg/mL. In addition, it did not show any cytotoxicity on RAW 264.7 macrophages at 100 microg/mL.
Georg Thieme Verlag KG Stuttgart, New York.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abies / chemistry*
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Cell Line
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Crystallography, X-Ray
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Flavonoids / isolation & purification
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Flavonoids / pharmacology*
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Inhibitory Concentration 50
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Lipopolysaccharides
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Macrophages / drug effects*
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Magnetic Resonance Spectroscopy
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Mice
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Nitric Oxide / biosynthesis*
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Plant Components, Aerial
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Spiro Compounds / isolation & purification
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Spiro Compounds / pharmacology*
Substances
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Anti-Inflammatory Agents
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Flavonoids
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Lipopolysaccharides
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Plant Extracts
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Spiro Compounds
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larixinol
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Nitric Oxide