Abstract
This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / enzymology*
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Circular Dichroism
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Hydrolases / chemistry
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Hydrolases / genetics
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Hydrolases / isolation & purification*
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Hydrolases / metabolism*
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Molecular Sequence Data
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Mycobacterium bovis / enzymology
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Protein Multimerization
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Protein Structure, Tertiary
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Sequence Analysis, DNA
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Substrate Specificity
Substances
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DNA, Bacterial
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Hydrolases
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haloalkane dehalogenase
Associated data
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GENBANK/AM696288
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GENBANK/AM696289