In vitro cytotoxicity of orthodontic primers

Prog Orthod. 2009;10(1):4-11.
[Article in English, Italian]

Abstract

Objectives: The aim of this study was to evaluate the cytotoxicity of four orthodontic primers: Transbond XT and Transbond MIP (3M, USA), Eagle Fluorsure (American Orthodontics, USA) and Ortho Solo (Ormco, USA).

Materials and methods: Balb 3T3 cells were exposed to different concentrations of primers (0-0.25 mg/ml). Mitochondrial dehydrogenase activity was evaluated by MTT assay and cell necrosis was measured by flow cytometry (propidium iodide staining).

Results: All the materials decreased cell viability in a dose related manner. Cytotoxicity of orthodontic primers based on concentrations which caused a 50% decrease of mitochondrial activity was ranked as follows: Transbond XT (45.57 mg/ml) > Eagle Fluorsure (49.27 mg/ml) > Transbond MIP (64.35 mg/ml) > Ortho solo (70.09 mg/ml).

Conclusions: Our results suggest that the cytotoxic potencies demonstrated by orthodontic primers might be of clinical relevance since they disturbed cell metabolism and induced cell death in monolayer cultures.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • BALB 3T3 Cells
  • Cell Death / drug effects
  • Cell Membrane / drug effects
  • Cell Survival / drug effects
  • Coloring Agents
  • Dentin-Bonding Agents / toxicity*
  • Dose-Response Relationship, Drug
  • Ethanol / toxicity
  • Flow Cytometry
  • Materials Testing
  • Methacrylates / toxicity
  • Mice
  • Mitochondria / enzymology
  • Necrosis
  • Oxidoreductases / drug effects
  • Resin Cements / toxicity*
  • Tetrazolium Salts
  • Thiazoles

Substances

  • Coloring Agents
  • Dentin-Bonding Agents
  • Methacrylates
  • OrthoSolo
  • Resin Cements
  • Tetrazolium Salts
  • Thiazoles
  • Transbond XT
  • Transbond moisture-insensitive primer
  • Ethanol
  • Oxidoreductases
  • thiazolyl blue