Effect of serum from mice immunized with group A streptococcus antigens on the efficiency of in vitro cloning of stromal precursor cells
Bull Exp Biol Med. 2008 Dec;146(6):756-8.
doi: 10.1007/s10517-009-0380-y.
[Article in
English,
Russian]
Affiliation
- 1 Laboratory of Immunity Regulation, N. F. Gamaleya Institute of Epidemiology and Microbiology, the Russian Academy of Medical Sciences, Moscow, Russia. [email protected]
Abstract
The efficiency of cloning of stromal precursor cell in mouse bone marrow culture increases significantly (2-3-fold) in the presence of serum from mice immunized with type 5 group A streptococcus antigens (5-20 microl serum/ml culture medium) in comparison with intact animal serum. The levels of TNF-alpha and IFN-gamma are significantly reduced (2.7 times and more than 6-fold, respectively) in the sera of immunized mice in comparison with normal serum. Serum levels of IL-2, -4, -5, -10, and -12 were about the same in both groups; no granulocyte-macrophage CSF was detected. These data attest to appreciable effect of immunization with streptococcal antigens on the bone marrow stromal tissue; this effect is presumably mediated through serum cytokines.
MeSH terms
-
Animals
-
Antigens, Bacterial / immunology*
-
Bone Marrow Cells / cytology*
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Cells, Cultured
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Clone Cells
-
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
-
Guinea Pigs
-
Interferon-gamma / metabolism
-
Interleukin-10 / metabolism
-
Interleukin-12 / metabolism
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Interleukin-2 / metabolism
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Interleukin-4 / metabolism
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Interleukin-5 / metabolism
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Mice
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Mice, Inbred CBA
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Serum / immunology*
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Serum / metabolism*
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Streptococcus pyogenes / immunology*
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Stromal Cells / cytology*
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Antigens, Bacterial
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Interleukin-2
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Interleukin-5
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Interleukin-12
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Interleukin-4
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Interferon-gamma
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Granulocyte-Macrophage Colony-Stimulating Factor