Abstract
Genetically prepared protein A fusion proteins, having retained antibody binding capacity, were used to design different well-defined standard molecular weight marker proteins for Western blotting. The blotted marker proteins are developed at the same time and with the same reagents as the protein sample of interest.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / standards
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Blotting, Western / standards*
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Escherichia coli / genetics
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Luciferases / genetics
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Luciferases / standards
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Molecular Weight
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Proteins / genetics
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Proteins / standards*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / standards
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Reference Standards
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Staphylococcal Protein A / chemistry
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Staphylococcal Protein A / genetics*
Substances
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Proteins
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Recombinant Fusion Proteins
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Staphylococcal Protein A
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Luciferases
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Alkaline Phosphatase