Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography

Krystyna Skalicka-Woźniak; Tomasz Mroczek; Ian Garrard; Kazimierz Głowniak

doi:10.1016/j.chroma.2009.05.077

Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography

J Chromatogr A. 2009 Jul 24;1216(30):5669-75. doi: 10.1016/j.chroma.2009.05.077. Epub 2009 Jun 2.

Authors

Krystyna Skalicka-Woźniak¹, Tomasz Mroczek, Ian Garrard, Kazimierz Głowniak

Affiliation

¹ Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland. [email protected]

PMID: 19523639
DOI: 10.1016/j.chroma.2009.05.077

Abstract

A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds--alsaticol and alsaticocoumarin A. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1) was developed. Compounds were obtained from the dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. Optimized HSCCC offers a rapid method of obtaining new natural compounds.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Apiaceae / chemistry*
Chromones / isolation & purification*
Countercurrent Distribution / methods*
Fruit / chemistry*
Furocoumarins / isolation & purification*
Plant Extracts / isolation & purification

Substances

Chromones
Furocoumarins
Plant Extracts
dihydropyranochromone