Abstract
Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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Animals
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Chlorocebus aethiops
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DNA-Directed RNA Polymerases / genetics
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Genes, Reporter
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Genome, Viral
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Green Fluorescent Proteins / genetics
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Hepatitis Delta Virus / genetics*
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Humans
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Mice
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Plasmids
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Promoter Regions, Genetic
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RNA Polymerase I / genetics*
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RNA, Catalytic / genetics
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RNA, Viral / genetics
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Restriction Mapping
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Terminator Regions, Genetic
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Transcription, Genetic
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Vero Cells
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Virus Replication
Substances
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3' Untranslated Regions
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RNA, Catalytic
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RNA, Viral
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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DNA-Directed RNA Polymerases
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RNA Polymerase I