Frequent methylation of Vimentin in well-differentiated gastric carcinoma

Anticancer Res. 2009 Jun;29(6):2227-9.

Abstract

Background: Recently, it was shown that the vimentin gene, usually activated in mesenchymal cells, was highly methylated in colorectal carcinoma.

Materials and methods: The methylation status of the vimentin gene was examined in primary carcinomas and the corresponding normal tissues derived from 37 patients with gastric carcinoma using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.

Results: Aberrant methylation of the vimentin gene was detected in 14 out of 37 (38%) primary gastric carcinomas. This result suggested that the aberrant methylation of the vimentin gene was frequent in gastric carcinomas. Subsequently, clinicopathological data were correlated with the methylation score. A significant difference was observed in histology (p=0.0429). In addition, a trend was shown toward advancement of gastric carcinomas with vimentin methylation (p=0.0588).

Conclusion: In gastric carcinomas, well-differentiated adenocarcinoma was significantly methylated compared to poorly differentiated.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / secondary
  • Aged
  • Cell Differentiation*
  • DNA Methylation*
  • Female
  • Gastric Mucosa / metabolism
  • Gastric Mucosa / pathology
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lymphatic Metastasis
  • Male
  • Neoplasm Staging
  • Polymerase Chain Reaction
  • Prognosis
  • Promoter Regions, Genetic
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology*
  • Vimentin / genetics*

Substances

  • Vimentin