Mass spectrometry in high-throughput screening: a case study on acetyl-coenzyme a carboxylase using RapidFire--mass spectrometry (RF-MS)

Comb Chem High Throughput Screen. 2009 Sep;12(8):752-9. doi: 10.2174/138620709789104924. Epub 2009 Sep 1.

Abstract

In this review various technologies and approaches for the utilization of mass spectrometry in high-throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. The RapidFire mass spectrometry system, an integrated on-line solid-phase extraction system interfaced to a triple-quadrupole mass spectrometer is described in detail, and the identification of a series of inhibitors of the acetyl-coenzyme A carboxylase (ACC) assay is described.

Publication types

  • Review

MeSH terms

  • Acetyl-CoA Carboxylase / antagonists & inhibitors*
  • Acetyl-CoA Carboxylase / metabolism*
  • Drug Discovery
  • Enzyme Inhibitors / analysis*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • High-Throughput Screening Assays / methods*
  • Mass Spectrometry / methods*
  • Solid Phase Extraction

Substances

  • Enzyme Inhibitors
  • Acetyl-CoA Carboxylase