Abstract
The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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CD8-Positive T-Lymphocytes / classification
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CD8-Positive T-Lymphocytes / immunology
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Cell Separation
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Epitopes
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Flow Cytometry
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Fluorescent Dyes
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HLA-A Antigens / chemistry
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HLA-A2 Antigen
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Humans
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Immunologic Techniques* / statistics & numerical data
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Protein Structure, Quaternary
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Receptors, Antigen, T-Cell, alpha-beta / metabolism
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Sensitivity and Specificity
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Staining and Labeling / methods
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T-Lymphocyte Subsets / classification
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T-Lymphocyte Subsets / immunology*
Substances
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Epitopes
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Fluorescent Dyes
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HLA-A Antigens
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HLA-A*02:01 antigen
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HLA-A2 Antigen
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Receptors, Antigen, T-Cell, alpha-beta