Abstract
The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase-activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution
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Carboxypeptidases / antagonists & inhibitors*
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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GTPase-Activating Proteins / antagonists & inhibitors*
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Gene Expression Regulation, Fungal*
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Intracellular Signaling Peptides and Proteins
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Mutant Proteins / genetics
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Mutant Proteins / physiology
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Mutation, Missense
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / antagonists & inhibitors*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / physiology*
Substances
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Carrier Proteins
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GTPase-Activating Proteins
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IRA2 protein, S cerevisiae
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Intracellular Signaling Peptides and Proteins
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Mutant Proteins
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Saccharomyces cerevisiae Proteins
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Tfs1 protein, S cerevisiae
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Carboxypeptidases
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CPY protein, S cerevisiae