Abstract
The ability to elicit cross-neutralizing antibodies makes human papillomavirus (HPV) L2 capsid protein a possible HPV vaccine. We examined and compared the humoral response of mice immunized with a HPV-16 L2 DNA vaccine or with HPV-16 L2 protein. The L2 DNA vaccine elicited a non-neutralizing antibody response unlike the L2 protein. L2 DNA vaccination suppressed the growth of L2-expressing C3 tumor cells, which is a T cell mediated effect, demonstrating that the lack of non-neutralizing antibody induction by L2 DNA was not caused by lack of T cell immunogenicity of the construct.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Neutralizing / blood
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Antibodies, Viral / blood
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Capsid Proteins / immunology*
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Cell Line, Tumor
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Female
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Human papillomavirus 16 / immunology
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Mice
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Mice, Inbred C57BL
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Neutralization Tests
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Oncogene Proteins, Viral / immunology*
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Papillomavirus Infections / immunology
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Papillomavirus Infections / prevention & control*
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Papillomavirus Vaccines / immunology*
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Vaccines, DNA / immunology
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Capsid Proteins
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L2 protein, Human papillomavirus type 16
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Oncogene Proteins, Viral
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Papillomavirus Vaccines
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Vaccines, DNA