Simultaneous determination of dimethylamine, trimethylamine and trimethylamine-n-oxide in aquatic products extracts by ion chromatography with non-suppressed conductivity detection

J Chromatogr A. 2009 Jul 31;1216(31):5924-6. doi: 10.1016/j.chroma.2009.06.038. Epub 2009 Jun 18.

Abstract

An ion chromatography method with non-suppressed conductivity detection was developed for the simultaneous determination of dimethylamine (DMA), trimethylamine (TMA) and trimethylamine-n-oxide (TMAO) in aquatic products. They were separated by means of cation-exchange chromatography using a 3.0 mmol/L methanesulfonic acid solution as eluent and an IonPac CS17 column (250 mm x 4 mm i.d.) as the separation column. Detection limits of dimethylamine, trimethylamine and trimethylamine-n-oxide were 0.06, 0.08 and 0.10 mg/L, respectively. The relative standard deviations (RSDs) of peak area were less than 3.53%. The recoveries were between 93.7% and 104.1%. Unlike traditional methods, this validated method is inexpensive and stable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Ion Exchange / methods*
  • Dimethylamines / analysis*
  • Electric Conductivity
  • Linear Models
  • Methylamines / analysis*
  • Potassium / chemistry
  • Quaternary Ammonium Compounds / chemistry
  • Reproducibility of Results
  • Seafood / analysis*
  • Sensitivity and Specificity
  • Sodium / chemistry
  • Trichloroacetic Acid / chemistry

Substances

  • Dimethylamines
  • Methylamines
  • Quaternary Ammonium Compounds
  • Trichloroacetic Acid
  • Sodium
  • dimethylamine
  • trimethyloxamine
  • trimethylamine
  • Potassium