Grape-seed procyanidins modulate inflammation on human differentiated adipocytes in vitro

Cytokine. 2009 Aug;47(2):137-42. doi: 10.1016/j.cyto.2009.06.001. Epub 2009 Jun 27.

Abstract

Flavonoids are functional constituents of many fruits and vegetables. Procyanidins are flavonoids with an oligomeric structure, and it has been shown that they can improve the pathological oxidative state of a diabetic situation. To evaluate whether procyanidins can modulate inflammation, an event strongly associated with obesity, diabetes and insulin resistance states, we used human adipocytes (SGBS) and macrophage-like (THP-1) cell lines and administered an extract of grape-seed procyanidins (GSPE). THP-1 and SGBS cells pre-treated with GSPE showed a reduction of IL-6 and MCP-1 expression after an inflammatory stimulus. GSPE stimuli alone modulate adipokine (APM1 and LEP) and cytokine (IL-6 and MCP-1) gene expression. GSPE partially inhibited NF-kappaB translocation to the nucleus in both cell lines. These preliminary findings demonstrate that GSPE reduces the expression of IL-6 and MCP-1 and enhances the production of the anti-inflammatory adipokine adiponectin suggesting that may have a beneficial effect on low-grade inflammatory diseases such obesity and type 2 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / immunology*
  • Adipokines / metabolism
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Differentiation
  • Cell Line
  • Cytokines / metabolism*
  • Humans
  • Inflammation Mediators / metabolism
  • Macrophages / drug effects
  • Macrophages / immunology
  • NF-kappa B / metabolism
  • Plant Extracts / pharmacology
  • Proanthocyanidins / pharmacology*
  • Seeds / chemistry
  • Tumor Necrosis Factor-alpha / pharmacology
  • Vitis / chemistry*

Substances

  • Adipokines
  • Anti-Inflammatory Agents
  • Cytokines
  • Inflammation Mediators
  • NF-kappa B
  • Plant Extracts
  • Proanthocyanidins
  • Tumor Necrosis Factor-alpha