Abstract
The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC alpha encoding a protein of 421 amino acids (Mr 44,300) and lysC beta encoding a protein of 172 amino acids (Mr 18,600). The C. glutamicum aspartokinase was purified and found to contain two proteins of Mr 47,000 and Mr 18,000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Aspartate Kinase / genetics*
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Aspartate Kinase / metabolism
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Bacillus subtilis / genetics
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Corynebacterium / enzymology
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Corynebacterium / genetics*
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Escherichia coli / genetics
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Feedback
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Genes, Overlapping*
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Molecular Sequence Data
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Nucleic Acid
Substances
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Bacterial Proteins
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Recombinant Fusion Proteins
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Aspartate Kinase
Associated data
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GENBANK/D12728
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GENBANK/D12729
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GENBANK/D12730
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GENBANK/D12731
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GENBANK/D12732
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GENBANK/D12733
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GENBANK/S67471
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GENBANK/S67472
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GENBANK/S67544
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GENBANK/X55648
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GENBANK/X57226