High-performance liquid chromatographic separation and renaturation of protein kinase subunits: application to catalytic subunit of phosphorylase kinase

Methods Enzymol. 1991:200:436-43. doi: 10.1016/0076-6879(91)00160-x.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Chromatography, High Pressure Liquid / methods
  • Enzyme Activation
  • Escherichia coli / genetics
  • Kinetics
  • Macromolecular Substances
  • Muscles / enzymology*
  • Phosphorylase Kinase / genetics
  • Phosphorylase Kinase / isolation & purification*
  • Phosphorylase Kinase / metabolism
  • Protein Kinases / isolation & purification*
  • Protein Kinases / metabolism
  • Rabbits
  • Recombinant Proteins / metabolism
  • Thermodynamics

Substances

  • Macromolecular Substances
  • Recombinant Proteins
  • Chloramphenicol O-Acetyltransferase
  • Protein Kinases
  • Phosphorylase Kinase