Emergence of invasive pneumococcal disease caused by multidrug-resistant serotype 19A among children in Barcelona

J Infect. 2009 Aug;59(2):75-82. doi: 10.1016/j.jinf.2009.05.012. Epub 2009 Jun 13.

Abstract

Objective: To describe the epidemiology of invasive pneumococcal disease (IPD) caused by Streptococcus pneumoniae serotype 19A.

Methods: We studied all children and adolescents with IPD caused by serotype 19A who were admitted to a Children's Hospital in Barcelona (1997-2007). Serotyping, antibiotic susceptibility and clonal analysis were performed.

Results: Comparing the pre-vaccine period (1997-2001) with the early vaccine period (2002-2004) and the late vaccine period (2005-2007) there was an increase of IPD caused by serotype 19A: 1 of 58 episodes (1.7%) vs. 8 of 54 episodes (14.8%) vs. 27 of 123 episodes (21.9%), respectively (P = 0.002). All S. pneumoniae serotype 19A isolated in the pre-vaccine and early vaccine periods (n = 9) were penicillin susceptible, while in the late vaccine period, 12 of 27 (44%) were penicillin nonsusceptible (P = 0.01). A clonal analysis revealed 15 different sequence types (STs) expressing serotype 19A. 10 of them were preexisting STs associated with serotype 19A including the multidrug-resistant ST320 and ST276.

Conclusion: There was an increase of IPD caused by S. pneumoniae serotype 19A which was mainly related with the emergence of preexisting clones several of them closely related with international multidrug-resistant clones. These results should be considered when selecting the new conjugate pneumococcal vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Typing Techniques
  • Child
  • Child, Preschool
  • Cluster Analysis
  • DNA Fingerprinting
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial*
  • Female
  • Genotype
  • Humans
  • Infant
  • Male
  • Microbial Sensitivity Tests
  • Pneumococcal Infections / epidemiology*
  • Pneumococcal Infections / microbiology*
  • Sequence Analysis, DNA
  • Serotyping
  • Spain / epidemiology
  • Streptococcus pneumoniae / classification*
  • Streptococcus pneumoniae / drug effects*
  • Streptococcus pneumoniae / isolation & purification

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial