Abstract
In this study, we observed and compared the effects of a high cholesterol diet (HCD) on cell proliferation and differentiation in the subgranular zone of the dentate gyrus of C57BL/6N (B6, susceptible strain) and C3H/HeN (C3H, resistant strain) mice. Ki67 (a marker for cell proliferation) positive cells) were significantly decreased in HCD-fed B6 mice compared to those in B6 (49.7%) and C3H mice fed a low cholesterol diet (LCD). In addition, doublecortin (DCX, a marker for cell differentiation or neuroblasts)-immunoreactive cells in HCD-fed B6 mice were significantly decreased compared to those in LCD-fed B6 and C3H mice. These results suggest that B6 strains are sensitive to HCD, which impairs cell proliferation and differentiation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Proliferation / drug effects
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Cholesterol, Dietary / administration & dosage*
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Cholesterol, Dietary / metabolism
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Dentate Gyrus / cytology*
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Dentate Gyrus / drug effects
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Dentate Gyrus / metabolism
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Doublecortin Domain Proteins
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Doublecortin Protein
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Female
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Hypercholesterolemia / metabolism
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Hypercholesterolemia / pathology
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Immunohistochemistry
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Ki-67 Antigen / metabolism
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Male
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Mice
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Microtubule-Associated Proteins / metabolism
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Neurogenesis / physiology
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Neuropeptides / metabolism
Substances
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Cholesterol, Dietary
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Dcx protein, mouse
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Doublecortin Domain Proteins
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Doublecortin Protein
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Ki-67 Antigen
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Microtubule-Associated Proteins
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Neuropeptides