Abstract
A systematic phytochemical examination of the whole plant Dracocephalum forrestii led to the isolation of 4 new and 65 known chemical constituents. By detailed 1D and 2D NMR spectroscopic analyses, the new compounds were identified as 4-hydroxy-3-methoxyphenylethanol 8- O-[(6- O-syringoyl)- beta- D-glucopyranoside] (1), 3,4,5-trimethoxyphenylethanol beta- D-glucopyranoside ( 2), 4- O-[ beta- D-glucopyranosyl-(1 --> 3)- alpha- L-rhamnopyranosyl]phenylethylcinnamamide (3), and 9''- O- N-butyl lithospermate (4). The new isolates were evaluated for inhibitory activities against LPS-induced NO production in RAW 264.7 macrophages. Compound 2 revealed a moderate effect without any cytotoxicity under the assayed concentrations.
Georg Thieme Verlag KG Stuttgart. New York.
MeSH terms
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology
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Cell Line
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Cinnamates / chemistry
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Cinnamates / isolation & purification*
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Cinnamates / pharmacology
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Glucosides / chemistry
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Glucosides / isolation & purification*
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Glucosides / pharmacology
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Lamiaceae / chemistry*
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Lipopolysaccharides
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Molecular Structure
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Nitric Oxide / antagonists & inhibitors*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
Substances
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4-O-(beta-D-glucopyranosyl-(1- 3)-alpha-L-rhamnopyranosyl)phenylethylcinnamamide
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4-hydroxy-3-methoxyphenylethanol 8-O-((6-O-syringoyl)-beta-D-glucopyranoside)
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Anti-Inflammatory Agents
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Cinnamates
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Glucosides
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Lipopolysaccharides
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Plant Extracts
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Nitric Oxide