Abstract
The purpose of this study was to assess tendon metabolism and suture pull-out strength after simple tendon suture in a tissue culture model. One hundred and twelve flexor digitorum profundus tendons from 28 dogs were cultured for 7, 14, or 21 days with or without a static tensile load. In both groups increased levels of matrix metalloproteinase (MMP) mRNA was noted. Suture pull-out strength did not decrease during tissue culture. While the presence of a static load had no effect on the pull-out strength, it did affect MMP mRNA expression. This tissue culture model could be useful in studying the effect of factors on the tendon-suture interface.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Collagen Type I / genetics
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Collagen Type I / metabolism
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Collagen Type III / genetics
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Collagen Type III / metabolism
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Dogs
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Forelimb*
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Matrix Metalloproteinases / genetics
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Matrix Metalloproteinases / metabolism
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sutures*
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Tendons / metabolism*
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Tendons / physiopathology*
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Tendons / surgery
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Tensile Strength
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Tissue Culture Techniques
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Tissue Inhibitor of Metalloproteinases / genetics
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Tissue Inhibitor of Metalloproteinases / metabolism
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Weight-Bearing
Substances
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Collagen Type I
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Collagen Type III
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinases
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Matrix Metalloproteinases