Abstract
Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Birds
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Genotype
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Humans
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Influenza A virus / classification*
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Influenza A virus / genetics
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Influenza A virus / isolation & purification*
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Influenza in Birds / diagnosis
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Influenza in Birds / virology*
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Influenza, Human / diagnosis
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Influenza, Human / virology
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Neuraminidase / genetics*
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Oligonucleotide Array Sequence Analysis / methods*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Viral Proteins / genetics*
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Virology / methods*
Substances
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Viral Proteins
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NA protein, influenza A virus
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Neuraminidase