Response of human bone marrow stromal cells to a resorbable P(2)O(5)-SiO(2)-CaO-MgO-Na(2)O-K(2)O phosphate glass ceramic for tissue engineering applications

Acta Biomater. 2010 Feb;6(2):598-606. doi: 10.1016/j.actbio.2009.07.017. Epub 2009 Jul 17.

Abstract

This work focuses on the synthesis and characterization of a novel bioresorbable glass ceramic phosphate-based material (GC-ICEL). More specifically, its solubility in different aqueous media (water, Tris-HCl and acellular simulated body fluid) and the response of human stromal cells cultured on it were investigated. X-ray diffraction analysis showed the presence of two crystalline phases identified as Na(2)Mg(PO(4))(3) and Ca(2)P(2)O(7) and dissolution tests highlighted a preferential dissolution of the Na(2)Mg(PO(4))(3) phase and of the residual amorphous phase in all the chosen media. Soaking tests in simulated body fluid showed precipitation of a hydroxyapatite layer, demonstrating the bioactivity of GC-ICEL, which is partially due to the reported bioactivity of Ca(2)P(2)O(7). The effect of GC-ICEL on adhesion, proliferation and osteoblastic gene expression of human bone marrow-derived stromal cells was also studied. Combining molecular and biochemical analyses, it was found that bone marrow cell differentiation was stimulated over proliferation on GC-ICEL. Moreover, the expression of bone-related genes in cells cultured on GC-ICEL confirmed the bioactivity of this phosphate-based glass ceramic, which might have a stimulatory effect on osteogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Bone Marrow Cells / cytology*
  • Cell Adhesion
  • Cell Proliferation
  • Ceramics*
  • DNA Primers
  • Glass*
  • Humans
  • Stromal Cells / chemistry*
  • Tissue Engineering*
  • X-Ray Diffraction

Substances

  • DNA Primers