Resolution-recovery for EPR imaging of free radical molecules in mice

Magn Reson Med. 2009 Sep;62(3):788-95. doi: 10.1002/mrm.22029.

Abstract

A method of post-processing to enhance the image resolution of the distribution of free radical molecules obtained with continuous-wave electron paramagnetic resonance (CW-EPR) imaging is reported. The low spatial resolution of EPR imaging, which has created difficulties in biomedical applications, was overcome by the method of resolution-recovery for EPR imaging. High spatial resolution images for the distribution of free radical molecules with a very short relaxation time were obtained with this method. The method's two-step postprocessing consists of conventional deconvolution and filtered back-projection and a process of iterative deconvolution. The resolution-recovery method was demonstrated with three-dimensional (3D) imaging of stable nitroxyl radicals in mouse head. In phantom experiments with a solution of triarylmethyl (TAM) radicals, the spatial resolution was improved by a factor of 7 with the resolution-recovery method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Animals
  • Electron Spin Resonance Spectroscopy / methods*
  • Free Radicals / analysis*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Tissue Distribution
  • Whole Body Imaging / methods*

Substances

  • Free Radicals