Molecular and epidemiological features of GIIb norovirus outbreaks in Victoria, Australia, 2002-2005

J Med Virol. 2009 Sep;81(9):1652-60. doi: 10.1002/jmv.21582.

Abstract

The epidemiology of GIIb norovirus outbreaks and the characteristics of GIIb open reading frame (ORF) 2 recombinant forms are poorly understood and this study examined these questions using norovirus-associated gastroenteritis outbreaks in Victoria, Australia, during 2002-2005. Twenty-one GIIb outbreaks were detected and were the second most common ORF 1 norovirus outbreak genotype (5%) after GII.4 (90%). Both GIIb and GII.4 outbreaks peaked in warmer months of the year but their periodicity was different. ORF 2 sequencing analysis was carried out in the two regions previously designated C and D. RT-PCR region D primers were less sensitive than region C primers. No evidence of recombination between regions C and D was found. ORF 2 genotypes for the 21 GIIb outbreaks were: GII.1 (10 outbreaks), GII.3 (10 outbreaks) and, apparently for the first time, GII.13 (1 outbreak). GIIb outbreaks could occur in a broad range of settings and there was no correlation between ORF 2 genotype and setting except that all 5 outbreaks involving mainly young children were associated with GIIb/GII.3.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Caliciviridae Infections / epidemiology*
  • Caliciviridae Infections / virology*
  • Child
  • Child, Preschool
  • Disease Outbreaks*
  • Genotype
  • Humans
  • Infant
  • Middle Aged
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Norovirus / classification*
  • Norovirus / genetics*
  • Norovirus / isolation & purification
  • Phylogeny
  • Seasons
  • Sequence Analysis, DNA
  • Sequence Homology
  • Victoria / epidemiology
  • Viral Proteins / genetics

Substances

  • Viral Proteins

Associated data

  • GENBANK/FJ972694
  • GENBANK/FJ972695
  • GENBANK/FJ972696
  • GENBANK/FJ972697
  • GENBANK/FJ972698
  • GENBANK/FJ972699
  • GENBANK/FJ972700
  • GENBANK/FJ972701
  • GENBANK/FJ972702
  • GENBANK/FJ972703
  • GENBANK/FJ972704
  • GENBANK/FJ972705
  • GENBANK/FJ972706
  • GENBANK/FJ972707
  • GENBANK/FJ972708
  • GENBANK/FJ972709
  • GENBANK/FJ972710
  • GENBANK/FJ986373
  • GENBANK/FJ986374
  • GENBANK/FJ986375
  • GENBANK/FJ986376
  • GENBANK/FJ986377
  • GENBANK/FJ986378
  • GENBANK/FJ986379
  • GENBANK/FJ986380
  • GENBANK/FJ986381
  • GENBANK/FJ986382
  • GENBANK/FJ986383
  • GENBANK/FJ986384
  • GENBANK/FJ986385
  • GENBANK/FJ986386
  • GENBANK/FJ986387
  • GENBANK/FJ986388