Backbone 1H, 13C and 15N assignments of a 59 kDa Salmonella typhimurium periplasmic oligopeptide binding protein, OppA

Kaori Kurashima-Ito; Kayano Moromisato; Kaoru Nishimura; Markus Wälchli; Jeremy R H Tame; Yutaka Ito

doi:10.1007/s12104-007-9008-1

Backbone 1H, 13C and 15N assignments of a 59 kDa Salmonella typhimurium periplasmic oligopeptide binding protein, OppA

Biomol NMR Assign. 2007 Jul;1(1):37-9. doi: 10.1007/s12104-007-9008-1. Epub 2007 May 30.

Authors

Kaori Kurashima-Ito¹, Kayano Moromisato, Kaoru Nishimura, Markus Wälchli, Jeremy R H Tame, Yutaka Ito

Affiliation

¹ Research Group for Bio-supramolecular Structure-Function, RIKEN, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.

PMID: 19636820
DOI: 10.1007/s12104-007-9008-1

Abstract

Salmonella typhimurium OppA is the periplasmic oligopeptide-binding protein. Backbone resonances of OppA(D419N) on its own were assigned for approximately 90% of residues. Missing residues are localised around the ligand-binding site, suggesting conformational flexibility in the unliganded state.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Carrier Proteins / chemistry*
Carrier Proteins / genetics
Carrier Proteins / metabolism
Lipoproteins / chemistry*
Lipoproteins / genetics
Lipoproteins / metabolism
Molecular Structure
Mutagenesis, Site-Directed
Nuclear Magnetic Resonance, Biomolecular
Oligopeptides / metabolism
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Salmonella typhimurium / chemistry*
Salmonella typhimurium / genetics

Substances

Bacterial Proteins
Carrier Proteins
Lipoproteins
Oligopeptides
Recombinant Proteins
oligopeptide-binding protein, bacteria