NMR assignment of the nonstructural protein nsp3(1066-1181) from SARS-CoV

Biomol NMR Assign. 2008 Dec;2(2):135-8. doi: 10.1007/s12104-008-9104-x. Epub 2008 Jul 22.

Abstract

Sequence-specific NMR assignments of the globular core comprising the residues 1066-1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [(13)C, (15)N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the (13)C chemical shifts.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbon Isotopes / chemistry
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Sequence Data
  • Molecular Weight
  • Nitrogen Isotopes / chemistry
  • Protein Structure, Tertiary
  • Protons
  • RNA-Dependent RNA Polymerase / chemistry*
  • Viral Nonstructural Proteins / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Protons
  • Viral Nonstructural Proteins
  • Nsp3 protein, SARS-CoV
  • RNA-Dependent RNA Polymerase