1H, 13C, and 15N assignment of the oxidized and reduced forms of T. brucei glutathione peroxidase-type tryparedoxin peroxidase

Johannes Melchers; Luise Krauth-Siegel; Claudia Muhle-Goll

doi:10.1007/s12104-008-9086-8

1H, 13C, and 15N assignment of the oxidized and reduced forms of T. brucei glutathione peroxidase-type tryparedoxin peroxidase

Biomol NMR Assign. 2008 Jun;2(1):65-8. doi: 10.1007/s12104-008-9086-8. Epub 2008 Apr 10.

Authors

Johannes Melchers¹, Luise Krauth-Siegel, Claudia Muhle-Goll

Affiliation

¹ Biochemie-Zentrum der Universität Heidelberg, Im Neuenheimer Feld 504, 69120 Heidelberg, Germany.

PMID: 19636927
DOI: 10.1007/s12104-008-9086-8

Abstract

The cysteine-homologues of glutathione peroxidases in Trypanosoma brucei catalyze the trypanothione/tryparedoxin-dependent reduction of hydroperoxides. We report the 1H, 13C, and 15N assignment of the oxidized and reduced form of the enzyme by NMR. Major changes between these two forms were only observed for residues close to the catalytic site.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Carbon Isotopes / chemistry
Glutathione Peroxidase / chemistry*
Magnetic Resonance Spectroscopy / methods*
Molecular Sequence Data
Molecular Weight
Nitrogen Isotopes / chemistry
Oxidation-Reduction
Peroxidases / chemistry*
Protons
Protozoan Proteins / chemistry*
Trypanosoma brucei brucei / enzymology*

Substances

Carbon Isotopes
Nitrogen Isotopes
Protons
Protozoan Proteins
Peroxidases
tryparedoxin peroxidase
Glutathione Peroxidase