1H, 15N, 13C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase

Daniel R Perez; Gerhard Wider

doi:10.1007/s12104-009-9158-4

1H, 15N, 13C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase

Biomol NMR Assign. 2009 Jun;3(1):133-6. doi: 10.1007/s12104-009-9158-4. Epub 2009 Apr 7.

Authors

Daniel R Perez¹, Gerhard Wider

Affiliation

¹ Institute of Molecular Biology and Biophysics, ETH Zurich, 8093, Zurich, Switzerland.

PMID: 19636964
DOI: 10.1007/s12104-009-9158-4

Abstract

Acyl carrier proteins participate in the synthesis of fatty acids. Here we report the NMR resonances assignment of the acyl carrier protein domain of the Saccharomyces cerevisiae fatty acid synthase which corresponds to the fragment 138A-302L in the primary structure. The assignment will allow performing NMR studies with the aim to investigate the intrinsic dynamics of this protein, and to study the structural changes upon apo-holo transformation in order to unveil the mechanism of binding of the growing acyl chain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyl Carrier Protein / chemistry*
Amino Acid Sequence
Carbon Isotopes / chemistry
Fatty Acid Synthases / chemistry*
Magnetic Resonance Spectroscopy / methods*
Molecular Sequence Data
Nitrogen Isotopes / chemistry
Protein Structure, Tertiary
Protein Subunits
Protons
Saccharomyces cerevisiae Proteins / chemistry*

Substances

Acyl Carrier Protein
Carbon Isotopes
Nitrogen Isotopes
Protein Subunits
Protons
Saccharomyces cerevisiae Proteins
Fatty Acid Synthases
FAS1 protein, S cerevisiae