Half-life mapping of nitroxyl radicals with three-dimensional electron paramagnetic resonance imaging at an interval of 3.6 seconds

Anal Chem. 2009 Sep 1;81(17):7501-6. doi: 10.1021/ac901169g.

Abstract

This technical note reports a continuous-wave electron paramagnetic resonance (CW-EPR) imager that can visualize the distribution of free radicals with a half-life of subminutes in three-dimensional (3D) space. A total of 46 EPR spectra under magnetic field gradients, called projections, were obtained for image reconstruction at an interval of 3.6 s. A shortened data-acquisition time was achieved with the use of analog signals that drove field gradient coils in the imager. 3D mapping of the half-lives of nitroxyl radicals (4-hydroxyl-2,2,6,6-tetramethyl-piperidinyl-1-oxyl) was demonstrated in their reduction reaction with ascorbic acid. Inhomogeneous half-lives were clearly mapped pixel-by-pixel in a sample tube.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbic Acid / metabolism
  • Electron Spin Resonance Spectroscopy / economics
  • Electron Spin Resonance Spectroscopy / instrumentation
  • Electron Spin Resonance Spectroscopy / methods*
  • Equipment Design
  • Half-Life
  • Imaging, Three-Dimensional / economics
  • Imaging, Three-Dimensional / instrumentation
  • Imaging, Three-Dimensional / methods*
  • Nitrogen Oxides / analysis*
  • Nitrogen Oxides / metabolism
  • Oxidation-Reduction
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Nitrogen Oxides
  • nitroxyl
  • Ascorbic Acid