Abstract
Four peptides corresponding to highly conserved motives within the first two RNA recognition motif-type domains of ELAV proteins were prepared, and their effect on the stability of NOVA-1 and VEGF ELAV-target mRNAs was evaluated. Biological results show that in the presence of phorbol 12-myristate 13-acetate (a PKC activator triggering the ELAV pathway), an equimolar mixture of peptides induces a statistically significant stabilization of the selected transcripts, suggesting a synergic effect of the two stimuli.
MeSH terms
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Amino Acid Motifs
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Antigens, Neoplasm / genetics*
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Cell Line, Tumor
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Conserved Sequence
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ELAV Proteins / chemistry*
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Enzyme Activators / pharmacology
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Humans
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Nerve Tissue Proteins / genetics*
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Neuro-Oncological Ventral Antigen
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Oligopeptides / chemical synthesis*
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Oligopeptides / chemistry
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Oligopeptides / pharmacology
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Protein Kinase C / metabolism
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RNA Stability / drug effects*
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RNA, Messenger / metabolism*
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RNA-Binding Proteins / genetics*
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Tetradecanoylphorbol Acetate / pharmacology
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Vascular Endothelial Growth Factor A / genetics*
Substances
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Antigens, Neoplasm
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ELAV Proteins
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Enzyme Activators
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Nerve Tissue Proteins
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Neuro-Oncological Ventral Antigen
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Oligopeptides
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RNA, Messenger
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RNA-Binding Proteins
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Vascular Endothelial Growth Factor A
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Protein Kinase C
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Tetradecanoylphorbol Acetate