Abstract
T-cell epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) glycoproteins 4 (GP4), 5 (GP5) and nucleocapsid (N) were predicted using bioinformatics and later tested by IFN-gamma ELISPOT in pigs immunized with either a modified live vaccine (MLV) or DNA (open reading frames 4, 5 or 7). For MLV-vaccinated pigs, immunodominant epitopes were found in N but T-epitopes were also found in GP4 and GP5. For DNA-immunized pigs, some peptides were differently recognized. Using a large set of PRRSV sequences it was shown that N contains a conserved epitope and that for GP5, the genotype-I counterparts of previously reported epitopes of genotype-II strains were also immunogenic.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Computational Biology
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Epitopes, T-Lymphocyte / genetics*
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Epitopes, T-Lymphocyte / immunology*
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Interferon-gamma / metabolism
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Leukocytes, Mononuclear / immunology
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Nucleocapsid Proteins / genetics*
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Nucleocapsid Proteins / immunology*
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Porcine respiratory and reproductive syndrome virus / genetics
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Porcine respiratory and reproductive syndrome virus / isolation & purification*
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Swine
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Vaccines, Attenuated / immunology
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Vaccines, DNA / immunology
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Viral Envelope Proteins / genetics*
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Viral Envelope Proteins / immunology*
Substances
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Epitopes, T-Lymphocyte
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Nucleocapsid Proteins
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Vaccines, Attenuated
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Vaccines, DNA
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Viral Envelope Proteins
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Interferon-gamma