Abstract
To investigate the role of detoxification-related liver genes in amnesic shellfish poisoning toxin metabolism, red sea bream Pagrus major were exposed to domoic acid (DA, 2mugg(-1) wet weight) for 24h. Hepatic mRNA expression levels of AHR, ARNT, CYP1 and GSTs were determined by semi-quantitative RT-PCR. The cytosolic factors aryl hydrocarbon receptor (AHR) and aryl hydrocarbon receptor nuclear translocator (ARNT) mRNA levels of DA exposure group were substantially enhanced by 113.3% and 90.9%, respectively. Consistent with this result, the phase I xenobiotic metabolizing enzyme (XME) cytochrome P-450 1A (CYP1A) was significantly induced. In contrast, the transcriptions of three major phase II XME glutathione S-transferases as well as heat shock protein 70 were not significantly affected by DA exposure. These results suggest a possible role of CYP1A after DA exposure in the toxin metabolism of marine fish, possibly through the AHR/ARNT signaling pathway.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amnesia / chemically induced
-
Amnesia / metabolism
-
Animals
-
Aryl Hydrocarbon Receptor Nuclear Translocator / classification
-
Aryl Hydrocarbon Receptor Nuclear Translocator / genetics
-
Aryl Hydrocarbon Receptor Nuclear Translocator / metabolism
-
Cloning, Molecular
-
Cytochrome P-450 CYP1A1 / classification
-
Cytochrome P-450 CYP1A1 / genetics
-
Cytochrome P-450 CYP1A1 / metabolism*
-
Kainic Acid / analogs & derivatives*
-
Kainic Acid / toxicity
-
Liver / drug effects*
-
Liver / enzymology
-
Liver / metabolism
-
Marine Toxins / toxicity*
-
Phylogeny
-
RNA, Messenger / metabolism
-
Receptors, Aryl Hydrocarbon / metabolism
-
Sea Bream / genetics
-
Sea Bream / metabolism*
-
Shellfish Poisoning
-
Transcription Factors / metabolism*
-
Transcription, Genetic / drug effects
Substances
-
Marine Toxins
-
RNA, Messenger
-
Receptors, Aryl Hydrocarbon
-
Transcription Factors
-
Aryl Hydrocarbon Receptor Nuclear Translocator
-
Cytochrome P-450 CYP1A1
-
domoic acid
-
Kainic Acid