Abstract
A simple and rapid analytical method is described for the simultaneous quantitative analysis of three different N-acylethanolamides in human biological samples: anandamide (AEA), oleoylethanolamide (OEA), and palmitoylethanolamide (PEA). The method is based on a new hybrid solid phase extraction-precipitation technology followed by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS) analysis using d(4)-AEA as the internal standard. The method is linear up to 100ng/ml with a limit of quantitation of 50pg/ml for AEA and 100pg/ml for OEA and PEA. Good reproducibility, accuracy, and precision were demonstrated during the method validation. Application of this new methodology to the analysis of clinical study samples is presented.
MeSH terms
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Adolescent
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Adult
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Amides
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Amidohydrolases / antagonists & inhibitors
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Arachidonic Acids / analysis*
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Arachidonic Acids / blood
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Calibration
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Cannabinoids
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Chromatography, High Pressure Liquid / methods
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Endocannabinoids
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Enzyme Inhibitors / administration & dosage
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Enzyme Inhibitors / pharmacology
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Ethanolamines
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Female
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Humans
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Leukocytes, Mononuclear / chemistry*
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Male
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Middle Aged
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Molecular Structure
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Oleic Acids / analysis*
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Oleic Acids / blood
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Palmitic Acids / analysis*
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Palmitic Acids / blood
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Polyunsaturated Alkamides / analysis*
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Polyunsaturated Alkamides / blood
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Reproducibility of Results
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Semen / chemistry*
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Solid Phase Microextraction / methods
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Tandem Mass Spectrometry / methods
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Young Adult
Substances
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Amides
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Arachidonic Acids
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Cannabinoids
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Endocannabinoids
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Enzyme Inhibitors
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Ethanolamines
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Oleic Acids
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Palmitic Acids
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Polyunsaturated Alkamides
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virodhamine
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oleoylethanolamide
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palmidrol
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Amidohydrolases
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fatty-acid amide hydrolase
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anandamide