Prenyltransferase substrate binding pocket flexibility and its application in isoprenoid profiling

Mol Biosyst. 2009 Sep;5(9):913-7. doi: 10.1039/b902370d. Epub 2009 Mar 26.

Abstract

This communication explores prenyltransferase substrate binding pocket flexibility to tag and enrich isoprenoids using affinity-based purification for metabolomic studies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / metabolism
  • Binding Sites
  • Dimethylallyltranstransferase / chemistry
  • Dimethylallyltranstransferase / metabolism*
  • Diphosphates / metabolism
  • Diterpenes / metabolism
  • Kinetics
  • Metabolomics / methods*
  • Naphthoquinones / metabolism
  • Pliability
  • Prenylation
  • Protein Binding
  • Reproducibility of Results
  • Streptomyces / enzymology
  • Terpenes / metabolism*

Substances

  • Bacterial Proteins
  • Diphosphates
  • Diterpenes
  • Naphthoquinones
  • Terpenes
  • geranyl diphosphate
  • naphterpin B
  • Dimethylallyltranstransferase