Molecular cloning and characterization of LPS-binding protein/bactericidal permeability-increasing protein (LBP/BPI) from olive flounder, Paralichthys olivaceus

Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):256-63. doi: 10.1016/j.vetimm.2009.07.010. Epub 2009 Jul 30.

Abstract

A lipopolysaccharide (LPS)-binding protein/bactericidal permeability-increasing protein (LBP/BPI) homolog was isolated from peripheral blood leukocytes cDNA library of olive flounder Paralichthys olivaceus. The isolated LBP/BPI cDNA is 2806bp in length with a 1419bp open reading frame (ORF) that encodes a protein of 472 amino acid residues. The LPS-binding domain is well conserved in the N-terminal barrel, showing high sequence identities with other teleost LBP/BPI as well as those of mammals. RT-PCR analysis revealed that mRNA expression of LBP/BPI was significantly elevated in all tested tissues (liver, gill, intestine, head kidney, and spleen) after intraperitoneal injection of the gram-negative bacterium Edwardsiella tarda or the gram-positive bacterium Streptococcus iniae. This expression pattern profile corresponded to that of acute inflammatory cytokines, suggesting that it plays a role in the innate immune response, in particular, the acute phase response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Proteins / chemistry
  • Acute-Phase Proteins / genetics*
  • Acute-Phase Reaction / genetics
  • Acute-Phase Reaction / immunology
  • Amino Acid Sequence
  • Animals
  • Antimicrobial Cationic Peptides / chemistry
  • Antimicrobial Cationic Peptides / genetics*
  • Base Sequence
  • Blood Proteins / chemistry
  • Blood Proteins / genetics*
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Edwardsiella tarda / immunology
  • Edwardsiella tarda / pathogenicity
  • Fish Proteins / chemistry
  • Fish Proteins / genetics*
  • Flounder / genetics*
  • Flounder / immunology*
  • Immunity, Innate / genetics
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Streptococcus / immunology
  • Streptococcus / pathogenicity
  • Tissue Distribution

Substances

  • Acute-Phase Proteins
  • Antimicrobial Cationic Peptides
  • Blood Proteins
  • Carrier Proteins
  • DNA, Complementary
  • Fish Proteins
  • Membrane Glycoproteins
  • bactericidal permeability increasing protein
  • lipopolysaccharide-binding protein