Objectives: Preparation of reusable and easy to handle Celite chromatographic columns.
Design and methods: Weighting precise Celite quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite under negative pressure.
Results: These new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good.
Conclusions: The reusable Celite cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite minicolumns.