Abstract
These experiments were designed to evaluate the role of cytokines in early T cell development within the thymus. By using a thymic organ culture model, we have studied the influence of high dose of IL-2 (10 to 1000 IU/ml) on the cell populations that are generated during 12 days starting from a thymic rudiment of 14-day-old mouse embryo. The IL-2 treatment resulted in the expansion of Thy-1+/-, CD4-, CD8-, CD3-, Fc gamma RII+, CD5 (Lyt-1)-, HSA-, Pgp- 1+, Mel-14- population. These cells had the morphology of large granular lymphocytes and displayed broad cytotoxic activity. In addition, IL-2-treated organ cultures had a dramatic decrease in CD4+CD8+ thymocytes, a marked reduction in TCR-alpha beta+ thymocytes--even more pronounced in the TCR-V beta 6+ and TCR-V beta 8+ thymocytes--and no significant changes in the number of TCR-gamma delta+ as compared to control organ cultures.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD*
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Antigens, Differentiation / analysis
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Antigens, Differentiation, T-Lymphocyte / analysis
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Antigens, Ly / analysis
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Antigens, Surface / analysis
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CD3 Complex
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CD4 Antigens / analysis
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Cell Differentiation / drug effects
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Cytotoxicity, Immunologic / drug effects
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Dose-Response Relationship, Drug
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Flow Cytometry
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Interleukin-2 / pharmacology*
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Membrane Glycoproteins*
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Mice
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Mice, Inbred BALB C
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Organ Culture Techniques
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Receptors, Antigen, T-Cell / analysis
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Receptors, Antigen, T-Cell / classification
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Receptors, Antigen, T-Cell / metabolism
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Receptors, Fc / analysis
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Receptors, IgG
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Tetraspanin 29
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Thy-1 Antigens
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Thymus Gland / cytology*
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Thymus Gland / embryology
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Thymus Gland / immunology
Substances
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Antigens, CD
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Antigens, Differentiation
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Ly
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Antigens, Surface
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CD3 Complex
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CD4 Antigens
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Cd9 protein, mouse
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Interleukin-2
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Membrane Glycoproteins
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Receptors, Antigen, T-Cell
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Receptors, Fc
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Receptors, IgG
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Tetraspanin 29
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Thy-1 Antigens