Synthesis and biological evaluation of a novel cardiolipin affinity matrix

Org Biomol Chem. 2009 Sep 21;7(18):3691-7. doi: 10.1039/b909306k. Epub 2009 Jul 14.

Abstract

Cardiolipin (1) is a dimeric phospholipid found in the mitochondrial membranes of both plants and animals. In order to understand better its role, we report the preparation of an immobilised analogue (2) using phosphoramidite chemistry; the probe has been used successfully to bind a recombinant protein containing a cardiolipin-binding domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiolipins / chemistry*
  • Cardiolipins / metabolism*
  • Organophosphorus Compounds / chemistry
  • Protein Kinase C / chemistry
  • Protein Kinase C / metabolism
  • Protein Structure, Tertiary
  • Rats
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sepharose

Substances

  • Cardiolipins
  • Organophosphorus Compounds
  • Recombinant Proteins
  • phosphoramidite
  • Sepharose
  • protein kinase N
  • Protein Kinase C