Disruption of transepithelial resistance by enterotoxigenic Escherichia coli

Amber M Johnson; Radhey S Kaushik; Philip R Hardwidge

doi:10.1016/j.vetmic.2009.08.020

Disruption of transepithelial resistance by enterotoxigenic Escherichia coli

Vet Microbiol. 2010 Feb 24;141(1-2):115-9. doi: 10.1016/j.vetmic.2009.08.020. Epub 2009 Aug 20.

Authors

Amber M Johnson¹, Radhey S Kaushik, Philip R Hardwidge

Affiliation

¹ Department of Veterinary Science, South Dakota State University, Brookings, SD 57007, USA.

PMID: 19733985
DOI: 10.1016/j.vetmic.2009.08.020

Abstract

Transepithelial resistance and tight junction protein localization in porcine intestinal epithelial cells were evaluated as a function of infection with porcine enterotoxigenic Escherichia coli isolates differing in adhesin and enterotoxin profiles. Robust heat-labile enterotoxin-independent reduction of host transepithelial resistance was observed in the absence of tight junction protein mislocalization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
Cell Line
Enterotoxigenic Escherichia coli / physiology*
Epithelial Cells / microbiology*
Intestines / cytology
Intestines / microbiology
Microscopy, Fluorescence
Swine
Tight Junctions / microbiology