Molecular analysis of the IGHA and MHC class III region genes in one family with IgA and C4 deficiencies

Exp Clin Immunogenet. 1990;7(3):170-80.

Abstract

We report molecular studies in 2 IgA-deficient persons. One of them had an unusual association with an acute lymphoblastic leukaemia; his sister was also IgA deficient and shared an HLA haplotype and a complotype known to be associated to IgA deficiencies. The 2 IgA-deficient siblings also had low C4 serum levels due to C4A*Q0 allele. We showed that both defects were transmitted independently in the family. Molecular analysis revealed no major structural defects of the IGHA coding and switch regions, whereas a broad C4A-21-OHA deletion was responsible for the C4A*Q0 phenotype. These results confirm previous data showing that IgA deficiencies seem to be, in most cases, a regulatory defect rather than a structural defect of the coding IGHA region itself. These data were further supported by another molecular study in a patient with a recurrent Landry-Guillain-Barre syndrome who showed total absence of serum IgA and sIgA+B cells with no major structural defect of the IGHA region.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Blotting, Southern
  • Complement C4 / deficiency*
  • Complement C4 / genetics
  • DNA Probes
  • Dysgammaglobulinemia / genetics*
  • Genomic Library
  • Humans
  • IgA Deficiency*
  • Immunoglobulin A / genetics
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Switch Region / genetics
  • Major Histocompatibility Complex / genetics
  • Male
  • Middle Aged
  • Pedigree
  • Polymorphism, Restriction Fragment Length

Substances

  • Complement C4
  • DNA Probes
  • Immunoglobulin A
  • Immunoglobulin Heavy Chains