Abstract
There are few studies that have examined the frequencies of epitope-specific CD4(+) T cells following the use of a highly effective vaccine, yet such data would potentially be of value for the development of novel vaccination strategies. In this study we tracked human epitope-specific CD4(+) T cell responses over time after immunisation with a live attenuated varicella zoster virus vaccine by MHC Class II tetrameric complexes and functional assays. We show that the peptide-specific responses reflect those against whole virus antigens, and are similar in both frequency and phenotype to those found in healthy volunteers, despite a highly attenuated and clinically inapparent infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antibodies, Viral / blood
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Antigens, Viral / immunology
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CD4-Positive T-Lymphocytes / immunology*
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Chickenpox / prevention & control*
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Chickenpox Vaccine / immunology*
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Epitopes, T-Lymphocyte / immunology*
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Female
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HLA-DR Antigens / immunology
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HLA-DRB1 Chains
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Health Personnel
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Humans
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Immunity, Cellular
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Immunoglobulin G / blood
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Interferon-gamma / immunology
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Leukocytes, Mononuclear / immunology
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Male
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Middle Aged
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Vaccines, Attenuated / immunology
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Young Adult
Substances
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Antibodies, Viral
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Antigens, Viral
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Chickenpox Vaccine
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Epitopes, T-Lymphocyte
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HLA-DR Antigens
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HLA-DRB1 Chains
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HLA-DRB1*15:01 antigen
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Immunoglobulin G
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Vaccines, Attenuated
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Interferon-gamma