Synthesis of exciton-controlled fluorescent probes for RNA imaging

Nucleic Acids Symp Ser (Oxf). 2009:(53):155-6. doi: 10.1093/nass/nrp078.

Abstract

The excitonic interaction of thiazole orange dyes is known to suppress fluorescence emission. We have developed hybridization-sensitive fluorescent probes utilizing the excitonic interaction of two thiazole orange dyes connected to the probes. Here, we report nuclease-resistant hybridization-sensitive probes for long-term intracellular RNA imaging.

MeSH terms

  • Fluorescent Dyes / chemical synthesis*
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • RNA / analysis*
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • RNA