Cell-targeted self-assembled DNA nanostructures

J Am Chem Soc. 2009 Oct 14;131(40):14237-9. doi: 10.1021/ja9015638.

Abstract

We present two strategies for attaching self-assembled DNA arrays to the surfaces of cells. Our first approach uses biotin-streptavidin interactions to bind DNA architectures to biotinylated cells. The second approach takes advantage of specific antibody-cell surface interactions, conjugated arrays and the subsequent binding to native epidermal growth factor receptors expressed on cancer cells. DNA array-cell surface interactions were visualized by fluorescence, confocal microscopy, and scanning electron microscopy. This novel application of DNA nanoarrays provides strategies to specifically label cell surfaces with micrometer-sized patches, bind cells onto a designed functionalized DNA scaffold, engineer cell/cell networks into microtissues, and deliver materials to cell surfaces.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / immunology
  • Antibodies / metabolism
  • Biotin / chemistry
  • DNA, Single-Stranded / chemical synthesis
  • DNA, Single-Stranded / chemistry*
  • DNA, Single-Stranded / metabolism
  • ErbB Receptors / immunology
  • ErbB Receptors / metabolism
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Jurkat Cells
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Microscopy, Fluorescence
  • Models, Molecular
  • Nanostructures / chemistry*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Streptavidin / chemistry

Substances

  • Antibodies
  • DNA, Single-Stranded
  • Fluorescent Dyes
  • Biotin
  • Streptavidin
  • ErbB Receptors