Abstract
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism
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Carrier Proteins / antagonists & inhibitors
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Chlorine / physiology
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Cloning, Molecular
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GABA Plasma Membrane Transport Proteins
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Gene Expression
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Membrane Transport Proteins*
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Microinjections
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Molecular Sequence Data
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Nerve Tissue Proteins / antagonists & inhibitors
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Oocytes / metabolism
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Organic Anion Transporters*
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Poly A / analysis
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RNA, Messenger / analysis
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Rats
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Sodium / physiology
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Structure-Activity Relationship
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Xenopus
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gamma-Aminobutyric Acid / metabolism*
Substances
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Carrier Proteins
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GABA Plasma Membrane Transport Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Organic Anion Transporters
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RNA, Messenger
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Slc6a1 protein, rat
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Poly A
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Chlorine
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gamma-Aminobutyric Acid
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Sodium