Association of DLC1 gene polymorphism with susceptibility to hepatocellular carcinoma in Chinese hepatitis B virus carriers

Cancer Epidemiol. 2009 Oct;33(3-4):265-70. doi: 10.1016/j.canep.2009.08.011. Epub 2009 Sep 17.

Abstract

Background: Lost or downexpression of the gene deleted in liver cancer 1 (DLC1) has been implicated in the development of hepatocellular carcinoma (HCC). We examined the relationship between DLC1 polymorphisms and HCC risk among Chinese.

Methods: Three DLC1 polymorphisms, Ex11+255T>G (rs3739298), Ex11-620G>A (rs532841) and IVS19+108C>T (rs621554), were genotyped in 434 patients with HCC and 480 controls by PCR-RFLP. The associations with the susceptibility to HCC were evaluated while controlling for confounding factors.

Results: We observed significantly increased susceptibility to HCC for the C/C genotype compared with T/T of IVS19+108C>T in the HBV carriers (OR=2.95, 95% CI, 1.65-5.26, P<0.001). Compared with the haplotype G-A-T (in order of Ex11+255T>G, Ex11-620G>A and IVS19+108C>T), the haplotype T-G-C was also significantly associated with an increased susceptibility to HCC among HBV carriers (OR=2.16, 95% CI, 1.08-4.35, P=0.009). The stratified analysis indicated no modification of the confounding factors on the increased susceptibility to HCC related to the DLC1 polymorphism/haplotype.

Conclusions: Our findings suggest that DLC1 genetic polymorphism or haplotype play a role in mediating the susceptibility to HBV-related HCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / virology
  • Carrier State
  • China
  • Female
  • GTPase-Activating Proteins
  • Genetic Predisposition to Disease
  • Genotype
  • Haplotypes
  • Hepatitis B virus / isolation & purification*
  • Humans
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / virology
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide
  • Tumor Suppressor Proteins / genetics*

Substances

  • DLC1 protein, human
  • GTPase-Activating Proteins
  • Tumor Suppressor Proteins