Verapamil and cyclosporin A potentiate the effects of chemotherapeutic drugs in the human medullary thyroid carcinoma TT cell line not expressing the 170 kDa P-glycoprotein

Cancer Lett. 1990 Nov 5;54(3):125-31. doi: 10.1016/0304-3835(90)90033-t.

Abstract

The TT-cell line, derived from a patient with metastatic medullary thyroid carcinoma (MTC), was found to exhibit intrinsic resistance to vincristine (VCR) despite the absence of immunohistochemically detectable 170 kDa P-glycoprotein (PGP 170) associated with multidrug resistance (MDR). Verapamil and cyclosporin A, two well known resistance modifiers of MDR, were found to significantly potentiate the action of VCR (60-fold) and to a lesser degree also of VP-16 and daunorubicin (dnr). The present results suggests that resistance of MTC to chemotherapy may be at least partly circumvented by the addition resistance modifiers to chemotherapeutic regimens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Cyclosporins / pharmacology*
  • Daunorubicin / pharmacology
  • Dose-Response Relationship, Drug
  • Drug Resistance
  • Drug Synergism
  • Etoposide / pharmacology
  • Humans
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / physiology
  • Thyroid Neoplasms / drug therapy*
  • Thyroid Neoplasms / metabolism
  • Thyroid Neoplasms / pathology
  • Tumor Cells, Cultured
  • Verapamil / pharmacology*
  • Vincristine / pharmacology

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Cyclosporins
  • Membrane Glycoproteins
  • Vincristine
  • Etoposide
  • Verapamil
  • Daunorubicin