The gene for 17 beta-hydroxysteroid dehydrogenase maps to human chromosome 17, bands q12-q21, and shows an RFLP with ScaI

Hum Genet. 1990 Oct;85(5):473-6. doi: 10.1007/BF00194219.

Abstract

The gene encoding human 17 beta-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) is assigned to chromosome 17 by Southern blotting analyses of panels of human x rodent somatic cell hybrids and independently to 17q12-q21 using chromosomal in situ hybridization. A search for physical linkage between 17-HSD and the proto-oncogenes. THRA1 and ERBB2 (both reported to be located in this region of chromosome 17) was performed by pulsed-field gel electrophoresis (PFGE) using several rare-cutting restriction endonucleases. Because all three genes hybridized to DNA fragments of different lengths it seems unlikely that the gene for 17-HSD is located very close to THRA1 and ERBB2. Further evidence for this assumption was obtained from the absence of any coamplification of the 17-HSD gene in 9 breast tumors with amplification of the ERBB2 gene. Analyses of Southern blots of ScaI-digested DNAs from unrelated individuals from Northern Finland revealed a relatively infrequent diallelic restriction fragment length polymorphism, the allele frequencies of which were 0.04 (A1) and 0.96 (A2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 17-Hydroxysteroid Dehydrogenases / genetics*
  • Animals
  • Blotting, Southern
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 17*
  • Cricetinae
  • Cricetulus
  • DNA / metabolism
  • DNA Probes
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Hybrid Cells
  • Mice
  • Nucleic Acid Hybridization
  • Polymorphism, Restriction Fragment Length

Substances

  • DNA Probes
  • DNA
  • 17-Hydroxysteroid Dehydrogenases
  • endodeoxyribonuclease ScaI
  • Deoxyribonucleases, Type II Site-Specific