The chicken or the egg: microRNA-mediated regulation of mRNA translation or mRNA stability

Mol Cell. 2009 Sep 24;35(6):739-40. doi: 10.1016/j.molcel.2009.09.003.

Abstract

In this issue of Molecular Cell, Fabian et al. (2009) demonstrate that in cell-free extracts from mouse Krebs-2 ascites, microRNA-mediated translational repression precedes target mRNA deadenylation, and identify GW182, PABP, and deadenylase subunits CAF1 and CCR4 as factors required for deadenylation.

Publication types

  • Comment

MeSH terms

  • Animals
  • Argonaute Proteins
  • Ascites / genetics
  • Ascites / metabolism
  • Autoantigens / metabolism
  • Binding Sites
  • Carcinoma, Krebs 2 / genetics
  • Carcinoma, Krebs 2 / metabolism
  • Eukaryotic Initiation Factor-2 / metabolism
  • Eukaryotic Initiation Factor-4G / metabolism
  • Exoribonucleases
  • Gene Silencing*
  • Humans
  • Kinetics
  • Mice
  • MicroRNAs / metabolism*
  • Poly(A)-Binding Proteins / genetics
  • Poly(A)-Binding Proteins / metabolism*
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Proteins / genetics
  • Proteins / metabolism*
  • RNA Processing, Post-Transcriptional*
  • RNA Stability
  • RNA, Messenger / metabolism*
  • RNA-Induced Silencing Complex / genetics
  • RNA-Induced Silencing Complex / metabolism*
  • Receptors, CCR4 / metabolism
  • Repressor Proteins
  • Ribonucleases

Substances

  • Ago2 protein, mouse
  • Argonaute Proteins
  • Autoantigens
  • Ccr4 protein, mouse
  • Eukaryotic Initiation Factor-2
  • Eukaryotic Initiation Factor-4G
  • MicroRNAs
  • Poly(A)-Binding Proteins
  • Proteins
  • RNA, Messenger
  • RNA-Induced Silencing Complex
  • Receptors, CCR4
  • Repressor Proteins
  • mirnlet7 microRNA, mouse
  • Cnot7 protein, mouse
  • Exoribonucleases
  • Ribonucleases